The surgery involves connecting the mouth of one woman to the anus of the other, creating a horrific, symbiotic relationship. The two women are forced to endure the excruciating pain and humiliation of being connected in such a manner. As the story unfolds, Heiter kidnaps a third person, a Japanese tourist named Koji (Akihiro Kitamura), and adds him to the centipede, creating a three-person abomination.

"The Human Centipede" explores several themes, including the dangers of playing God, the consequences of unchecked ambition, and the degradation of human dignity. The film uses the centipede as a symbol of the monstrous "other," representing the fears and anxieties associated with bodily transformation and loss of control.

Regardless of one's opinion, "The Human Centipede" has left an indelible mark on the horror genre, pushing the boundaries of on-screen violence and exploring the darker aspects of human nature. As a cultural phenomenon, it continues to fascinate and repel audiences, serving as a testament to the power of cinema to challenge, disturb, and provoke.

Some critics praised the film for its boldness, originality, and technical achievements, while others condemned it as a gratuitous, exploitative, and misogynistic nightmare. The film's use of graphic violence, gore, and torture raised questions about the boundaries of on-screen violence and the limits of audience tolerance.

"The Human Centipede" is a film that polarizes audiences, sparking intense debate and discussion about its artistic merit, cultural significance, and moral implications. While some view it as a masterpiece of horror cinema, others condemn it as a gratuitous and exploitative exercise in shock value.

"The Human Centipede" has become a cult classic among horror fans, with a dedicated following and numerous references in popular culture. The film's iconic imagery, including the centipede's creation and the aftermath of the surgery, has been parodied and homaged in various forms of media.